Succinate dehydrogenase mutant of Rhizobium meliloti
نویسندگان
چکیده
منابع مشابه
Requirement of succinate dehydrogenase activity for symbiotic bacteroid differentiation of Rhizobium meliloti in alfalfa nodules.
Transmission electron microscopy was used to study the cellular morphologies of a wild-type Rhizobium meliloti strain (L5-30), a nitrogen fixation-ineffective (Fix-) succinate dehydrogenase mutant (Sdh-) strain, and a Fix+ Sdh+ revertant strain within alfalfa nodules and after free-living growth in a minimal medium containing 27 mM mannitol plus 20 mM succinate. The results showed a requirement...
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The synthesis o f /?-galactosidase in Rhizobium meliloti W U60 was found to be inducible by lactose and its non-metabolizable analogue, isopropyl-ß-D-thiogalactoside (IPTG). In contrast to Escherichia coli, galactose and melibiose were very weak inducers o f this enzyme in R. meliloti. The maximum level o f /3-galactosidase in this bacterium is 2% o f that in fully induced E. coli. In addition ...
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Transfer of an IncP plasmid carrying the Rhizobium meliloti nodFE, nodG, and nodH genes to Rhizobium trifolii enabled R. trifolii to nodulate alfalfa (Medicago sativa), the normal host of R. meliloti. Using transposon Tn5-linked mutations and in vitro-constructed deletions of the R. meliloti nodFE, nodG, and nodH genes, we showed that R. meliloti nodH was required for R. trifolii to elicit both...
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Transformation of R factor RP4 and its derivative pRK290 from Escherichia coli to Rhizobium meliloti is reported. The efficiency of transformation was in the range of 10(-5) per viable cell. In addition, chromosomal DNA prepared from one R. meliloti strain resistant to streptomycin was transferred to the isoleucine-valine-requiring mutant susceptible to streptomycin.
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A set of conserved, or common, bacterial nodulation (nod) loci is required for host plant infection by Rhizobium melioti and other Rhizobium species. Four such genes, nodDABC, have beeii indicated in R. meliloti 021'by genetic analysis and DNA sequencing. An essential step toward Iunderstanding the function of these genes is to characterize their protein products. We used in vitro and maxicell ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1982
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.151.3.1621-1623.1982